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np ibv  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology np ibv
    Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus <t>NP</t> <t>(NP(IAV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP <t>(NP(IBV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).
    Np Ibv, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/np ibv/product/Santa Cruz Biotechnology
    Average 92 stars, based on 4 article reviews
    np ibv - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells"

    Article Title: Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells

    Journal: Journal of Virology

    doi: 10.1128/jvi.01901-23

    Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus NP (NP(IAV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP (NP(IBV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).
    Figure Legend Snippet: Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus NP (NP(IAV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP (NP(IBV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).

    Techniques Used: Infection, Activity Assay, Virus, Microscopy, Staining, Labeling



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    np ibv  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology np ibv
    Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus <t>NP</t> <t>(NP(IAV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP <t>(NP(IBV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).
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    Image Search Results


    Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus NP (NP(IAV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP (NP(IBV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).

    Journal: Journal of Virology

    Article Title: Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells

    doi: 10.1128/jvi.01901-23

    Figure Lengend Snippet: Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus NP (NP(IAV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP (NP(IBV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).

    Article Snippet: Briefly, cells grown on 18 mm round coverslips were fixed with 4% paraformaldehyde in PBS for 15 min at ambient temperature and permeabilized with cold methanol for 10 min. After 1 h blocking with 5% bovine serum albumin (BSA, BioShop, Burlington, ON, Canada) in PBS, staining was performed overnight at +40C with antibodies to the following targets: influenza A virus (IAV) polyclonal antibody (1:400, goat, Abcam, ab20841), NP (IAV) (1:1,000, mouse, Santa Cruz, sc-101352), NP (IBV) (1:200, mouse, Santa Cruz Biotechnology, sc-57885), PABPC1 (1:1000, rabbit, Abcam, ab21060), PABPN1 (1:200, rabbit, Abcam, ab75855), SON (1:600, rabbit, Abcam, ab121759), and SR proteins (1:100, mouse, Santa Cruz Biotechnology, sc-13509).

    Techniques: Infection, Activity Assay, Virus, Microscopy, Staining, Labeling